BRIGHT - Biodiversity Genomics Europe Workshop

BRIGHT - Biodiversity Research Integrating Barcoding, Genomics and High-throughput Technologies
Biodiversity Genomics Europe (BGE) Joint Network Training Workshop
Dates: 3-7 February 2025

Targeted audience: Polish students, young researchers, citizen scientists, stakeholders.

Knowledge requirement: Basic knowledge of biology, especially knowledge and interest in biodiversity, nucleic acids composition and function.

Costs: the course is free of charge (including coffee breaks); travel, accommodation and lunches are not covered

Language: English

Contact: polbol@biol.uni.lodz.pl

Online meetings assistance: Łukasz Trębicki (lukasz.trebicki@biol.uni.lodz.pl)

Form of participation:

1.       Practical + theoretical, in person (up to 20 participants)

2.      Theoretical only, online (up to 40 participants)

20 places are secured for in person participation in the course. The choice will be made based on your answers  in the registration form. The priority will be given to students, early carrier researchers, stake holders, citizen scientists.

Address: Banacha 12/16, 90-237 Łódź. Faculty of Biology and Environmental Protection, Department of Invertebrate Zoology and Hydrobiology, University of Lodz

Organizers: PolBOL UniLodz (polbol.uni.lodz.pl) and Biodiversity Genomics Europe consortium (biodiversitygenomics.eu)

Registration deadline: 18 December 2024.

Results of evaluation: 20 December 2024.

Thanks to the synergy of DNA barcoding, genomics methods and high-throughput sequencing technologies, recognition of biodiversity from individuals through populations to species levels became available for everyone. The workshop will provide practical training and theoretical knowledge on using modern molecular tools in a wide aspect of biodiversity studies.

Workshop covers (theoretical and practical):

  • Material sampling and storage

  • DNA isolation (barcoding and genomics)

  • DNA barcoding (amplification, quality control, sequencing with Oxford Nanopore Techniques)

  • Sequencing technologies and their applications

  • Genomic data applications and databases (reference genomes, conservation genomics)

  • Data analysis and management (databases: BOLD, ENA; bioinformatic pipelines)

  • Citizen Science

 

WORKSHOP HANDBOOK DOWNLOAD

 

Material for the course:

During the course we will focus on model invertebrate group - pollinators collected from Impatiens spp. from different sampling sites in Poland.

Genome: Gammarus sp. collected around the city of Lodz.


BGE training general schedule:

Monday, 3 Feb 2025

9:00-10:30

Welcome and morning coffee

Introduction of attendees and Instructors

10:30-10:45

Coffee break

10:45-12:00

DNA barcoding and BGE project (MG)

12:00-13:15

Lunch break

13:15-14:30

Planing DNA-based biodiversity study (PG)

14:30-14:45

Coffee break

14:45-16:30

eDNA in biodiversity assessment (KBS, AD)

16:30-16:45

Coffee break

16:45-18:00

Molecular lab class 0, DNA Isolation methods (TM)

 

Tuesday, 4 Feb 2025

9:00-10:30

Laboratory: DNA isolation for barcoding

10:30-10:45

Coffee break

10:45-12:00

BOLD database, sequence ID (TR)

12:00-13:15

Lunch break

13:15-14:30

Collecting material in practice (PG, GK)

14:30-14:45

Coffee break

14:45-16:30

Laboratory: High Molecular Weight (HMW) DNA isolation

16:30-16:45

Coffee break

16:45-18:00

BIOSCAN UK (LPC)

Barcoding with Nanopore (TM)

 

Wednesday, 5 Feb 2025

9:00-12:00

Laboratory: DNA amplification for Nanopore sequencing,

QC of HMW DNA 

12:00-13:15

Lunch break

13:15-14:30

DNA barcoding for fungi (IS)

14:30-14:50

Coffee break

14:50-17:50

Laboratory: DNA pooling, ONT library preparation, QC


Thursday, 6 Feb 2025

9:00-10:30

Computing: Large-scale barcoding using Nanopore sequencing (AS) 

10:30-10:50

Coffee break

10:45-12:00

Computing: ONTbarcoder Sequence ID (AS)

12:00-13:15

Lunch break

13:15-14:30

Museomics 101: How to harvest historical DNA from zoological collections? (MK)

14:30-14:45

Coffee break

14:45-16:30

FAIR data management, ENA (JP)

16:30-16:45

Coffee break

16:45-18:00

Reference genomes/ galaxy (TB)

 

Friday, 7 Feb 2025

9:00-10:30

Computing: Practical on genome assembly using Galaxy (TB), including coffee break

10:45-12:00

12:00-13:15

Lunch break

13:15-14:30

Visit in BioBank, library preparation for Illumina (MSK)

14:30-14:45

Coffee break

14:45-16:30

Computing: Practical on genome assembly using Galaxy (TB)

16:30-16:45

Coffee break

16:45-18:00

Citizen Science for biodiversity research (KBS)

Summary of the BRIGHT training

 

Lectures

Practical classes in wet laboratory

Practical classes outside or using computers

 

Instructors:

 

Theory:

Karolina Bącela-Spychalska (KBS), University of Lodz, Poland

Thomas Brown (TB), Leibniz Institute of Zoo and Wildlife Research, Germany 

Andrea Desiderato (AD), University of Lodz, Poland

Michał Grabowski (MG), University of Lodz, Poland

Piotr Gadawski (PG), University of Lodz, Poland

Marcin Kamiński (MK), Museum & Institute of Zoology, Polish Academy of Sciences, Poland

Tomasz Mamos (TM), University of Lodz, Poland

(JP), European Molecular Biology Laboratory, Hinxton, UK

Lyndall Pereira da Conceicoa (LPC), Wellcome Sanger Institute, UK

Tomasz Rewicz (TR), University of Lodz, Poland

Igor Siedlecki (IS), University of Warsaw, Poland

Amrita Srivathsan (AS), National History Museum, Germany

Grzegorz Tończyk (GT), University of Lodz, Poland

Marta Sobalska-Kwapis (MSK), University of Lodz, BioBank, Poland


Laboratory:

Karolina Biniek (KB), University of Lodz

Wiktoria Cechowicz (WC), University of Lodz

Piotr Gadawski (PG), University of Lodz

Gabriela Karlik (GK), University of Lodz

Tomasz Mamos (TM), University of Lodz

Serena Mucciolo (SM), University of Lodz

Tomasz Rewicz (TR), University of Lodz

 

Computing:

Thomas Brown (TB), Leibniz Institute of Zoo and Wildlife Research, Germany 

Andrea Desiderato (AD), University of Lodz

Tomasz Mamos (TM), University of Lodz

Amrita Srivathsan (AS), National History Museum, Berlin